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Vaginal shortening after surgical treatment of pelvic organ prolapse is associated with dyspareunia, which negatively affects women’s sexual life as well as their psychosocial well-being. The aim of the study is to determine the vaginal length in women with high-grade pelvic organ prolapse treated with laparoscopic sacropexy. In the prospective study we included 22 women with high-grade prolapse of the uterus or vagina that were treated. They underwent a gynaecological examination with a measurement of the vaginal length, as well as the evaluation of the degree of prolapse prior to the procedure (laparoscopic sacropexy). The second measurement and evaluation of the vaginal length during the follow-up examination between 6 to 12 weeks after surgery was done. The control group included 23 healthy women, without genital prolapse. There was no statistically significant difference in the mean vaginal length before and after surgery in the group of treated women.