https://doi.org/10.4081/uij.2011.e9

Morphological changes in the fast vs slow fiber profiles of the urethras of diabetic pregnant rats

Vol. 25 No. 1 (2011)
Submitted: 19 May 2011
Accepted: 11 October 2011
Published: 4 November 2011
diabetes, pregnancy, striated muscle fiber, urethra
Abstract Views: 801
PDF: 456
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Authors

Gabriela Marini
gacamarini@yahoo.com.br
Botucatu Medical School, UNESP - Univ Estadual Paulista, São Paulo, Brazil., Brazil.
Angélica M. Pascon Barbosa
Botucatu Medical School, UNESP - Univ Estadual Paulista, São Paulo, Brazil., Brazil.
Débora C. Damasceno
Botucatu Medical School, UNESP - Univ Estadual Paulista, São Paulo, Brazil., Brazil.
Selma M. Michelin Matheus
Botucatu Biosciences Institute, UNESP - Univ Estadual Paulista, São Paulo, Brazil., Brazil.
Rodrigo de Aquino Castro
São Paulo Federal University (UNIFESP), São Paulo, Brazil., Brazil.
Manoel João B. Castello Girão
São Paulo Federal University (UNIFESP), São Paulo, Brazil., Brazil.
Marilza V. Cunha Rudge
Botucatu Medical School, UNESP - Univ Estadual Paulista, São Paulo, Brazil., Brazil.
Background. This study was undertaken to test the hypothesis that diabetes and pregnancy detrimentally affect the normal function of urethral striated muscles in rats, providing a model for additional studies related to urinary incontinence. The aim of this study was to evaluate morphological alterations in the urethral striated muscles of diabetic pregnant rats. Design and methods. Twenty female Wistar rats were distributed into four experimental groups of five rats as follows: virgin, pregnant, diabetic virgin, and diabetic pregnant. Diabetes was induced using streptozotocin administration (40 mg/kg i.v.). The rats were lethally anesthetized, and the urethra and vagina were extracted as a unit. Cryostat sections (6 µm thick) were cut and stained with hematoxylin-eosin, and immunohistochemical procedures were performed and subjected to morphological and semi quantitative analysis. Results. The urethral striated muscle from the diabetic pregnant rats presented with the following variations: thinning and atrophy, disorganization and disruption associated with the colocalization of fast and slow fibers and a steady decrease in the proportion of fast vs slow fibers. Conclusion. Diabetes and pregnancy impair the urethral striated muscle and alter its fiber type distribution.

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Supporting Agencies

FAPESP (Process Number 2008/00989-4).
Gabriela Marini, Botucatu Medical School, UNESP - Univ Estadual Paulista, São Paulo, Brazil.
Experimental Research Laboratory, Department of Gynecology and Obstetrics
Angélica M. Pascon Barbosa, Botucatu Medical School, UNESP - Univ Estadual Paulista, São Paulo, Brazil.

Experimental Research Laboratory, Department of Gynecology and Obstetrics

Débora C. Damasceno, Botucatu Medical School, UNESP - Univ Estadual Paulista, São Paulo, Brazil.

Experimental Research Laboratory, Department of Gynecology and Obstetrics

Selma M. Michelin Matheus, Botucatu Biosciences Institute, UNESP - Univ Estadual Paulista, São Paulo, Brazil.
Department of Anatomy
Rodrigo de Aquino Castro, São Paulo Federal University (UNIFESP), São Paulo, Brazil.
Department of Gynecology
Manoel João B. Castello Girão, São Paulo Federal University (UNIFESP), São Paulo, Brazil.
Department of Gynecology
Marilza V. Cunha Rudge, Botucatu Medical School, UNESP - Univ Estadual Paulista, São Paulo, Brazil.

Experimental Research Laboratory, Department of Gynecology and Obstetrics

How to Cite

Marini, G., Pascon Barbosa, A. M., Damasceno, D. C., Michelin Matheus, S. M., de Aquino Castro, R., Castello Girão, M. J. B., & Cunha Rudge, M. V. (2011). Morphological changes in the fast vs slow fiber profiles of the urethras of diabetic pregnant rats. Urogynaecologia, 25(1), e9. https://doi.org/10.4081/uij.2011.e9

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